Analysis of 7-methylguanine using Isotope Dilution and Gas Chromatography/Electron Capture-Negative Chemical Ionization Mass Spectrometry

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Title:	Analysis of 7-methylguanine using Isotope Dilution and Gas Chromatography/Electron Capture-Negative Chemical Ionization Mass Spectrometry
Authors:	江素瑛(Chiang,Su-Yin);黃太鴻(Huang,Tai-Hung);(Shi-Nian Uang);(Hong-Dar Isaac Wu);(Yi-Chiech Wei);林慧怡;(James A Swenberg);(Kuen-Yuh Wu*)
Contributors:	中醫學院中國醫學研究所
Date:	2005-07
Issue Date:	2009-08-19 16:36:09 (UTC+8)
Abstract:	This paper presents results obtained for in vivo endogenous and exogenous 7-methylguanine (7-MG) analyzed using a method incorporating gas chromatography with electron-capture negative chemical ionization mass spectrometry and isotope dilution (GC/EC-ID-MS). 13C4-Labeled 7-MG was synthesized to serve as an internal standard to improve accuracy of quantitation, and was used to analyze 7-MG in livers of control mice and dacarbazine-treated mice. The results confirm that 7-MG in tissue DNA can be measured using this GC/EC-ID-MS method with excellent sensitivity and specificity. Administration of 0, 30, and 60 mg/kg dacarbazine to mice led to dose-dependent increases in the formation of 7-MG. The results indicate that this method could be applied to the analysis of endogenous and exogenous 7-MG in human tissues for future molecular epidemiology studies on potential health effects caused by methylating agents.
Relation:	RAPID COMMUNICATIONS IN MASS SPECTROMETRY19(14):1915~1920
Appears in Collections:	[Graduate Institute of Chinese Medical Science] Journal articles

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