Noninvasive nuclear factor-κB bioluminescence imaging for the assessment of host-biomaterial interaction in transgenic mice

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 中醫學院 > 中國醫學研究所 > 期刊論文 > Item 310903500/1194

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题名:	Noninvasive nuclear factor-κB bioluminescence imaging for the assessment of host-biomaterial interaction in transgenic mice
作者:	侯庭鏞(Tin-Yun Ho);陳悅生(Yueh-Sheng Chen);項千芸(Chien-Yun Hsiang)*
贡献者:	中醫學院中國醫學研究所
关键词:	Inflammation;Bioluminescence imaging;Genipin-cross-linked gelatin conduit;Nuclear factor-κB;Transgenic mice
日期:	2007-10
上传时间:	2009-08-19 16:36:05 (UTC+8)
摘要:	The inflammatory response is a key component in the biocompatibility of biomaterials. Among the factors that control the development of inflammation is a critical molecule nuclear factor-κB (NF-κB). Therefore, the aim of this study was to assess the feasibility of noninvasive whole-body real-time imaging for the evaluation of host–biomaterial interaction in the NF-κB transgenic mice. Transgenic mice, carrying the luciferase gene under the control of NF-κB, were constructed. In vivo bioluminescence imaging showed that the constitutive and induced NF-κB activities of transgenic mice were detected in most of the lymphoid tissues, demonstrating that NF-κB-driven luminescence reflected the inflammatory response in vivo. By the implantation of genipin-cross-linked gelatin conduit (GGC) and bacterial endotoxin-immersed GGC in the dorsal region, we detected a strong and specific luminescent signal from the tissue around the bacterial endotoxin-immersed GGC implant. Histological and immunohistochemical analysis also demonstrated that inflammation, characterized by the infiltration of immune cells, the accumulation of fluid, and the activation of NF-κB, was evoked around the same region. The correlation between the bioluminescence imaging and histological changes indicated that noninvasive imaging technique could be used to monitor the real-time inflammation in the implanted mice.
關聯:	BIOMATERIALS 28(30):4370~4377
显示于类别:	[中國醫學研究所] 期刊論文

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