丁酸梭菌益生菌於模擬腸胃道環境條件下之基本特性、病原菌拮抗性與安全性評估－生體外試驗模式

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Title:	丁酸梭菌益生菌於模擬腸胃道環境條件下之基本特性、病原菌拮抗性與安全性評估－生體外試驗模式
Authors:	曾政鴻(Tsen JH)
Contributors:	健康照護學院營養學系
Keywords:	丁酸梭菌;益生菌;耐酸;耐膽鹽;吸附性;拮抗能力;Clostridium butyricum, Probiotics, Acid tolerance, Bile tolerance, Adhesiveness, Antagonistic activity
Date:	2007-07-31
Issue Date:	2009-09-01 15:27:25 (UTC+8)
Abstract:	本研究在探討丁酸梭菌 (Clostridium butyricum)益生菌營養細胞與孢子型態菌體的胃酸與膽鹽耐性、腸道吸附性、拮抗病原菌之能力與安全性，並與Lactobacillus reuteri, Bifidobacterium bifidum就上述試驗性質進行比較，嘗試了解營養細胞與孢子形態的C. butyricum 對人體腸道中不良環境因子之抵抗性，以及拮抗C. perfringens的能力；同時探討該菌對人體腸道細胞之安全性，以評估C. butyricum 在保健食品上應用之可行性。酸與膽鹽耐受性，分別是以pH 2-4之酸液及0.1-0.4%之膽鹽溶液試驗之，而模擬腸道吸附性則是利用Caco-2 cells作為吸附試驗模式，抑菌試驗方面則以試驗菌株培養基上清液抑制C. perfringens生長之抑菌圈試驗為之。結果顯示在耐酸與耐膽鹽試驗方面，孢子形態的C. butyricum存活率顯著高於營養細胞形態者。而在模擬腸道細胞吸附試驗，則發現營養細胞形態的C. butyricum (70.5%)、L. reuteri (82.4%)和B. bifidum (77.8%)皆具有良好的吸附性，而孢子形態的C. butyricum則是呈低吸附性(8.2%)。抑菌圈試驗發現C. butyricum的培養基上清液可抑制C. perfringens的生長。此外，侵入性試驗也顯示C. butyricum具有安全性。 Spore-forming probiotic bacteria Clostridium butyricum was used to assess its in vitro potential probiotic properties of both spore and vegetative cell form. Tested items included acid (pH 2-4) tolerance, bile salts (0.1-0.4%) tolerance, adhesiveness and antagonistic effect on the pathogenic C. perfringens. Lactobacillus reuteri and Bifidobacterium bifidum were selected as reference strains for comparison in those mentioned characteristics. C. butyricum spore exhibited higher survival rate than its vegetative cells in acid and bile conditions. The vegetative cell of C. butyricum (70.5%), L. reuteri (82.4%) and B. bifidum (77.8%) possessed good adhesive properties. Nevertheless, C. butyricum spore showed low adhesiveness (8.2%). Spent culture supernatant (SCS) of C. butyricum could inhibit the growth of C. perfringens in the inhibition zone test. In vitro invasion assay also revealed the safety aspect of C. perfringens.
Appears in Collections:	[Graduate Institute of Nutrition ] Research reports

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