We evaluated peripheral nerve regeneration using biodegradable genipin-crosslinked gelatin nerve conduits (GGCs) with three different cross-linking degrees around 24%, 36%, and 51%. Biocompatibility and biodegradability of the GGC and its effectiveness as a guidance channel were examined as it was used to repair a 10 mm gap in the rat sciatic nerve. From this pilot study, we conclude that GGCs with the mean cross-linking degree at 36% can ensure nerve regeneration with a more mature structure demonstrated by better-developed epineurial and perineurial organization and axonal development. Regenerated nerves in the GGCs with the mean cross-linking degree at 24% and 51% were less favorable, could be due to degradation material irritation and mild nerve compression, respectivey.
