| 摘要: | 計畫之目的乃以大鼠藥動模式評估紫杉醇和黃芩、和甘草、和桔梗、和丹參、和六味地黃湯、和補中益氣湯的交互作用，以提供中西藥合併服用安全性的資料，也從這些單味藥或方劑中發掘一些成分，可能和抗癌西藥併用，以增加其療效，減少抗癌西藥的用量；除了可以節省醫療費用外，也可能降低癌症病人服用抗癌西藥的不適症狀，改善其生活品質，作為未來衛生主管機構對於中西藥併用於特殊疾病，如癌症用藥之參考資訊。以大鼠藥動的實驗結果看出，上述六種中藥，任一種於30分鐘前餵服，之後和口服紫杉醇沒有交互作用，但黃芩經十日，一天一次餵服後和口服紫杉醇出現交互作用，如增加餵服頻率至一天二次，十日後和口服紫杉醇出現顯著的(P ≤ 0.05)交互作用(增加紫杉醇Cmax、AUC)。黃芩的指標成分之一，黃芩苷，於30分鐘前或經十日，一天一次餵服後和口服紫杉醇都產生顯著的交互作用。黃芩或黃芩苷第一次被發現，在特定情形下和紫杉醇出現顯著的交互作用。黃芩為幾千年來使用的中藥，安全性高，未來能否成為合併紫杉醇治療的理想搭配有待更多的動物實驗結果。純化合物黃芩苷的交互作用結果，可提供更多機轉方面的資訊以解釋黃芩的功效。黃芩、桔梗、丹参、補中益氣湯和三種純化合物(黃芩苷、黃芩素、環孢靈) 均顯著地降低紫杉醇對抗藥性細胞株的 IC50。如何使用中藥以增加紫杉醇療效，減少紫杉醇用量有待更多的實驗結果。
The aim of the project is to use rat as a model to evaluate the effects of traditional Chinese Medicin (TCM) such as Huang Qin (Scutellerie baicalensis Georgi), Dan Shen, or licorice root etc on the pharmacokinetics (PK) of paclitaxel. Such TCM-drug interaction results can provide safety information when any of the above commonly used TCM is given together with anti-cancer drug, paclitaxel. At the mean time, it is possible to identify some active ingredients from the above Chinese medicines to potentiate the effectiveness of paclitaxel or lower its effective dosage, in order to save the chemotherapy cost, reduce the side effects caused by paclitaxel, and improve the quality of life of treated cancer patients.According to the rat PK results, any of the above TCM when given 30 minutes before the oral paclitaxel administration, no TCM-drug interaction was observed. When decoction of Huang qin was given once a day for 10 days, interaction was observed. When the feeding frequency increased to twice a day, significant (P ≤ 0.05) interaction was observed. For baicalin, no matter it was given 30 minutes before or once a day for 10 days, significant interaction was noticed. To our understanding, it is the first time that such results were reported. Huang qin has been used widely by Chinese for over a thousand years. Whether it can be used together with paclitaxel to improve its anticancer effects or bring benefit to cancer patients will be very interesting for us to study further. According to the results of cytotoxicity assay using drug resistant cell line, Huang Qin, JieGeng,Dan Shen, Bu Zhong Yi Qi, baicalin, baicalein, cyclosporine all significantly decreased the IC50 values of paclitaxel. |
