LUMICAN在近視的病程及病理變化中所扮演的角色

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題名:	LUMICAN在近視的病程及病理變化中所扮演的角色
其他題名:	The Role of Lumican in Progression and Pathogenesis of Myopia The Role of Lumican in Progression and Pathogenesis of Myopia
作者:	林慧茹(Hui-Ju Lin)
貢獻者:	中醫學院中醫學系學士班中醫內科學科;中國附醫眼科部
日期:	2009-07-31
上傳時間:	2009-09-01 13:38:18 (UTC+8)
摘要:	Lumican是鞏膜組織中一個主要的細胞外組成之一，鞏膜提供必要的眼球支撐，以維持眼壓及眼球的形狀 ﹔愈來愈多的證據顯示，改變鞏膜的組成是近視中調節眼軸長度變化的一個重要因素。本研究的目的在探討lumican在罹患近視及近視進行中所扮演的角色。本研究的第一部份，在探討lumican中已知及新的基因多型性與台灣人近視的關聯性。本研究的第二部分在探討這些基因多型性的功能，這是以表現型功能性質體的作用，藉由各種不同基因多型性在其中的表現，來比較這些不同的基因變異是否會有功能上的改變，及比較其造成改變的強弱。此外因為MAP kinase路徑主要能調節細胞的存活及凋亡，本研究也將了解lumican的基因轉錄在MAP kinase路徑中是否有調節作用，此外MAP kinase有三個主要的路徑(P38, JUN, ERK)也將分別加以探討。在第三部份，為了進一步了解lumican在活體內的作用，我們將構築基因轉殖的果蠅來表現 lumican基因，再以共焦顯微鏡及電子顯微鏡觀察其型態上的改變，以再做進一步的分析，並確認在體外功能性測試的實驗中所得到的改變。近視在台灣是一個嚴重的眼科疾病，台灣人近視的比例遠高於白種人，近視是造成視網膜病變感覺視力的一個重要因子，近視的研究可幫助我們找到避免及治療近視發生及進行的方法。 Lumican (LUM) is one of the major extracellular matrix components of sclera. The sclera provides the necessary mechanical support to counteract intraocular pressure and maintain normal shape of the eye. Increasing evidence suggests that changing the composition of the sclera is one of the major factors in regulating axial elongation of the eye as in myopia. Our aim was to examine the role of LUM in the susceptibility and progression of myopia. The first part of our study is detecting the relation between known and novel single nucleotide polymorphisms of LUM and high myopia patients in Taiwanese Chinese. In the second part, in order to compare the effect of function of different SNPs, we use functional assay to detect the LUM under the condition of site-directed mutation. Moreover, since MAP kinase pathway is important in survival and apoptosis of cells, we use luciferase reporter assay to detect the influences of the transcription of LUM in mitogen-activated protein (MAP) kinase pathway. There are three major pathways of MAP kinase (p38, JUN, ERK kinase pathway), they will be analysis individually. In the third part, in order to understand the function of LUM in vivo, transgenic drosophila is used to express the LUM. The phenotypes of transgenic drosophila are detected by confocal microscopy and electric microscopy. This may make us verify the results obtained from the functional assay and reporter assay in vitro. Myopia is an important eyes’ disease in Taiwan, the incidence of myopia in Taiwan is much higher than it in Caucasian. High myopia is an important etiology in retinopathy which can threaten the visual acuity. Study in myopia can help us in finding methods to prevent and teat the susceptibility and progression of high myopia
顯示於類別:	[中醫學系暨碩博班] 研究計畫

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