A potential role of YC-I on the inhibition of cytokine release in peripheral blood mononuclear leukocytes and endotoxemic mouse models

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Title:	A potential role of YC-I on the inhibition of cytokine release in peripheral blood mononuclear leukocytes and endotoxemic mouse models
Authors:	Pan, SL;Guh, JH;Peng, CY;Chang, YL;Cheng, FC;Chang, JH;Kuo, SC;Lee, FY;Teng, CM
Contributors:	藥學院藥化所;Natl Taiwan Univ, Coll Med, Inst Pharmacol, Taipei 10764, Taiwan;Natl Taiwan Univ, Coll Med, Sch Pharm, Taipei 10764, Taiwan;Yung Shin Pharmaceut Ind Co Ltd, Taichung, Taiwan;China Med Univ, Grad Inst Pharmaceut Chem, Taichung, Taiwan
Date:	2005
Issue Date:	2010-09-24 15:09:33 (UTC+8)
Publisher:	SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN
Abstract:	Pycnogenol, rich of many phytochemicals of medical value, is it commercialized nutrient supplement extracted from the bark of European coastal pine. In this study, we investigated the anti-tumor effects of Pycnogenol on HL-60, U937 and K562 human leukemia cell lines. We found that Pycnogenol inhibited cell proliferation dose- and time-dependently and the IC(50)s of Pycnogenol on HL-60, U937 and K562 cells were 150, 40 and 100 mu g/ml, respectively. When HL-60 cells were incubated with low concentrations of Pycnogenol (50, 100 and 125 mu g/ml) for 24 h. a prominent GO/G1 arrest was observed. followed by gradual accumulation of sub-G0/G1 nuclei. At 48 h of treatment, 50-70% of HL-60 cells differentiated, as evidenced by morphological changes, NBT reduction, induction of NSE activity, and increases of cell surface expression of CD11b. However. results front Annexin V/PI staining, DAPI staining and DNA fragmentation assay indicated that Pycnogenol induced HL-60. U937 and K562 cell apoptosis at their respective IC(50)s after 24 h of treatments. Pretreatment of z-DEVD-fmk, a caspase-3 specific inhibitor. not only decreased caspase-3 activity but also reduced the percentage of: apoptotic cells induced by Pycnogenol. This indicated that caspase-3 activation was involved in Pycnogenol induced-apoptosis. In conclusion, Pycnogenol induced differentiation and apoptosis in leukemia cells. Our data suggest that Pycnogenol could serve Lis a potent cancer chemopreventive or chemotherapeutic agent for human leukemia. (c) 2004 Elsevier Ltd. All rights reserved.
Relation:	THROMBOSIS AND HAEMOSTASIS 93(5):940-948
Appears in Collections:	[Graduate Institute of Pharmaceutical Chemistry] Journal articles

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