Expression of dihydrodiol dehydrogenase in the resected stage I non-small cell lung cancer

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Title:	Expression of dihydrodiol dehydrogenase in the resected stage I non-small cell lung cancer
Authors:	Chen, CY;Hsu, CP;Hsu, NY;Shih, CS;Lin, TY;Chow, KC
Contributors:	附設醫院醫研部;China Med Coll Hosp, Dept Med Res, Taichung 404, Taiwan;China Med Coll Hosp, Dept Pathol, Taichung 404, Taiwan;China Med Coll, Med Res Inst, Taichung, Taiwan;Chung Shun Med Coll, Taichung, Taiwan;Taichung Vet Gen Hosp, Taichung, Taiwan;Natl Yang Ming Univ, Taichung, Taiwan;China Med Coll Hosp, Div Chest Surg, Taichung 404, Taiwan
Date:	2002
Issue Date:	2010-09-24 14:57:01 (UTC+8)
Publisher:	PROFESSOR D A SPANDIDOS
Abstract:	We employed cDNA microarrays to identify the differentially expressed genes in a cisplatin-sensitive parental (2008) human ovarian carcinoma cell line and its cisplatin-resistant variant (2008/C13). Differential expression of five genes was found in the 2008/C13 cells, a result confirmed by semi-quantitative reverse transcription-PCR. The five genes were identified as fibroblast muscle-type tropomyosin and skeletal muscle-type tropomyosin, dihydrodiol dehydrogenase, apolipoprotein J and glucose-6-phosphate dehydrogenase variant-A. Treatment of the 2008 cells with cisplatin (at its IC50. concentration of 2 muM) induced expression of these genes, as determined by semi-quantitative reverse transcription-PCR analysis using gene-specific primers. In contrast, treatment of the drug-resistant 200S/C13* cells with cisplatin (at its IC50 concentration of 20 muM) did not lead to the induction of any of the aforementioned genes. Most importantly, constitutive overexpression of dihydrodiol dehydrogenase (but not the other genes) in the 2008 cells led to induction of cisplatin resistance, clearly indicating its role in the development of the resistance phenotype in the 2008/C13* cells. The development of cisplatin resistance in the transfected cells was associated with an increase in the dihydrodiol dehydrogenase enzyme activity. Although at present it is not clear how dihydrodiol dehydrogenase is involved in cisplatin resistance, the identification of this gene as a causal factor suggests the existence of a hitherto undefined pathway resulting in cisplatin resistance.
Relation:	ONCOLOGY REPORTS 9(3):515-519
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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