Effect of 5-methoxypsoralen (5-MOP) on cell apoptosis and cell cycle in human hepatocellular carcinoma cell line

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Title:	Effect of 5-methoxypsoralen (5-MOP) on cell apoptosis and cell cycle in human hepatocellular carcinoma cell line
Authors:	Lee, YM;Wu, TH;Chen, SF;Chung, JG
Contributors:	醫學院醫學系;China Med Coll, Dept Med, Taichung 400, Taiwan;Chungtai Inst Hlth Sci & Technol, Dept Med Technol, Taichung 406, Taiwan;China Jr Coll Med Technol, Dept Med Technol, Tainan 701, Taiwan
Date:	2003
Issue Date:	2010-09-24 14:50:37 (UTC+8)
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Abstract:	It is well known that N-acetyltransferase (NAT) plays an important role in the arylamine metabolism. We analysed the response of A549 human lung cancer cells for N-acetylation of 2-aminofluorene (AF) to curcumin. After curcumin treatment, the NAT activity was examined by HPLC, AF-DNA adduct formation was examined by HPLC, and NAT gene expression by polymerase chain reaction were detected. The NAT activity in the human A549 cells and cytosols was suppressed by curcumin in a dose-dependent manner. The results also demonstrated that gene expression (NAT1 mRNA) in human lung A549 tumor cells was inhibited and decreased by curcumin. After the incubation of human lung A549 tumor cells with AF with or without curcumin co-treatment, the cells were recovered and DNA was prepared and hydrolyzed to nucleotides. The adducted nucleotides were extracted into butanol and analyzation of AF-DNA adducts was done by HPLC. The results also demonstrated that curcumin decreases AF-DNA adduct formation in the human lung A549 tumor cells. (C) 2003 Elsevier Science Ltd. All rights reserved.
Relation:	TOXICOLOGY IN VITRO 17(3):279-287
Appears in Collections:	[醫學系] 期刊論文

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