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    題名: The role of the conserved histidine-aspartate pair in the 'base-off' binding of cobalamins
    作者: Chen, HP;Lung, FD;Yeh, CC;Chen, HL;Wu, SH
    貢獻者: 健康照護學院營養系;Natl Taipei Univ Technol, Inst Biotechnol, Taipei 106, Taiwan;Natl Taipei Univ Technol, Dept Chem Engn, Taipei 106, Taiwan;China Med Univ, Dept Nutr, Taichung 404, Taiwan;Nanjing Univ, Dept Chem, State Key Lab, Nanjing 210093, Peoples R China;Nanjing Univ, Dept Chem, Inst Coordinat Chem, Nanjing 210093, Peoples R China;Acad Sinica, Inst Biol Chem, Taipei 112, Taiwan
    日期: 2004
    上傳時間: 2010-09-24 14:34:40 (UTC+8)
    出版者: PERGAMON-ELSEVIER SCIENCE LTD
    摘要: The effects of ethanol extracts of three Chinese medicinal plants Dahuang (Rheum palmatum L.), Badou (Croton tiglium L.), and Huomaren (Cannabis sativa L.), on ion transport of the rat intestinal epithelia were studied. Rat intestinal epithelia mounted in an Ussing chamber attached with voltage/current clamp were used for measuring changes of the short-circuit current across the epithelia. The intestinal epithelia were activated with current raised by serosal administration of forskolin 5 mum. Ethanol extracts of the three plants all augmented the current additively when each was added after forskolin. In subsequent experiments, ouabain and bumetanide were added prior to ethanol extracts of these medicinal plants to determine their effect on Na+ and Cl- movement. The results suggest that ethanol extracts of the three medicinal plants may affect the Cl- movement more directly than Na+ movement in the intestinal epithelial cells. The results provide evidence for the pharmacologic mechanism of the three Chinese medicinal plants on the intestinal tract.
    關聯: BIOORGANIC & MEDICINAL CHEMISTRY 12(3):577-582
    顯示於類別:[營養學系暨碩士班 ] 期刊論文

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