Pre-tracheal lymph node metastasis in squamous cell carcinoma of the thoracic esophagus

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/28973

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题名:	Pre-tracheal lymph node metastasis in squamous cell carcinoma of the thoracic esophagus
作者:	Hsu, CP;Hsu, NY;Shai, SE;Hsia, JY;Chen, CY
贡献者:	附設醫院;Taichung Vet Gen Hosp, Div Thorac Surg, Dept Surg, Taichung 40705, Taiwan;Natl Yang Ming Univ, Sch Med, Taipei 112, Taiwan;China Med Univ Hosp, Taichung, Taiwan
日期:	2005
上传时间:	2010-09-24 14:17:31 (UTC+8)
出版者:	ELSEVIER SCI LTD
摘要:	Xanthomonas campestris pv. campestris (Xcc), a close relative to Pseudomonas aeruginosa, is the pathogen causing black rot in cruciferous plants. In P. aeruginosa, FleQ serves as a cognate activator of sigma(54) in transcription from several sigma(54)-dependent promoters of flagellar genes. These P. aeruginosa promoters have been analyzed for FleQ-binding sequences; however, no consensus was deduced. Xcc, although lacks fleSR, has a fleQ homologue residing among over 40 contiguously clustered flagellar genes. A fleQ mutant, Xc17fleQ, constructed by insertional mutation is deficient in FleQ protein, non-flagellated, and immobile. Transcriptional fusion assays on six. putative sigma(54)-dependent promoters of the flagellar genes, fliE, fliQ, fliL, flgG, flgB, and flhF, indicated that each of them is also FleQ dependent. Each of these promoters has a sequence with weak consensus to 5'-gaaacCCgccgCcgctTt-3', immediately upstream of the predicted sigma(54)-binding site, with an imperfect inverted repeat containing a GC-rich center flanked by several A and T at 5'- and 3'-ends, respectively. Replacing this region in fliE promoter with a HindIII recognition sequence abolished the transcription, indicating that this region responds to transcription activation by FleQ. (c) 2005 Elsevier Inc. All rights reserved.
關聯:	EJSO 31(7):749-754
显示于类别:	[台中附設醫院] 期刊論文

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