Molecular mechanisms of ZD1839-induced G1-cell cycle arrest and apoptosis in human lung adenocarcinoma A549 cells

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Title:	Molecular mechanisms of ZD1839-induced G1-cell cycle arrest and apoptosis in human lung adenocarcinoma A549 cells
Authors:	Chang, GC;Hsu, SL;Tsai, JR;Liang, FP;Lin, SY;Sheu, GT;Chen, CY
Contributors:	中醫學院中醫所;Chung Shan Med Univ, Inst Toxicol, Taichung, Taiwan;Taichung Vet Gen Hosp, Dept Internal Med, Div Pulm & Crit Care Med, Taichung, Taiwan;Taichung Vet Gen Hosp, Dept Educ & Res, Taichung, Taiwan;China Med Coll, Inst Chinese Med, Taichung, Taiwan;Taichung Vet Gen Hosp, Dept Surg, Div Thorac Surg, Taichung, Taiwan
Date:	2004
Issue Date:	2010-09-24 13:45:18 (UTC+8)
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Abstract:	Background. Our objective was to evaluate immune function after total parenteral nutrition (TPN) treatment in colorectal cancer patients. Methods. Data were collected from 11 patients with colorectal cancer who received total parenteral nutrition. Healthy medical staff volunteers were used as controls. Delay hypersensitivity skin test with eight agents tested was used before total parenteral nutrition (TPN) treatment. Phytohemagglutinin (PHA)-stimulated lymphocyte proliferation and mononuclear cytokine production (IL-1beta, IL-2, IFNgamma), serum ICAM-1 and IL-6, and lymphocyte subpopulation including CD4, CD8, and CD4/CD8 ratio were measured before and after TPN treatment. Results. Each patient had at least four of seven positive skin tests. After TPN treatment, lymphocyte proliferation increased remarkably (27,450 +/- 16,800 vs. 41,738 +/- 28,484, p = 0.0168) and showed no significant difference to control group (p = 0.0269). Levels of IL-1beta, IL-2, and IFNgamma after LPS-stimulation showed no significant difference before and after TPN treatment. Serum ICAM-1 showed a significant difference (388 +/- 158 vs. 481 +/- 210, p = 0.0023) before and after TPN treatment. IL-6 was significantly different between patient and control groups but not different between patients before and after TPN treatment. Conclusions. This study showed a significant increase of mitogen-stimulated lymphocyte proliferation and intercellular adhesion molecular 1 (ICAM-1) level after TPN supply. Adequate nutrition through TPN may augment immune function. (C) 2004 IMSS. Published by Elsevier Inc.
Relation:	BIOCHEMICAL PHARMACOLOGY 68(7):1453-1464
Appears in Collections:	[Graduate Institute of Chinese Medical Science] Journal articles

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